Abstract: Aristolochia triangularis Cham., is one of the most frequently used medicinal plant in Southern Brazil. Preparations containing the leaves and/or stems are traditionally used as anti-inflammatory, diuretic, as well as antidote against snakebites. This study screened A. triangularis extracts, fractions and isolated compounds for different bioactivities. A weak antiproliferative activity against human lung cancer cell line (A549) was observed only for chloroform fraction obtained from stems (CFstems - CC50: 2.93 µg/mL). Also, a moderate antimicrobial activity against Staphylococcus aureus was detected just for chloroform fraction obtained from leaves (CFleaves -13-16 mm inhibition zone). Additionally, two semi-purified fractions (CFstems-4 and CFleaves-4) selectively inhibited HSV-1 replication (IC50 values of 0.40 and 2.61 µg/mL, respectively), while only CFleaves showed promising results against Leishmania amazonensis. Fractionation of extracts resulted in the isolation of one neolignan (-) cubebin and one lignan (+) galbacin. However, these compounds are not responsible for the in vitro bioactivities herein detected. The presence of aristolochic acid I and aristolochic acid II in the crude ethanol extract of stems (CEEstems) and leaves (CEEleaves) was also investigated. The HPLC analysis of these extracts did not display any peak with retention time or UV spectra comparable to aristolochic acids I and II.

ABSTRACT Banara parviflora (A. Gray) Benth. is a species that belongs to Salicaceae family and is native to Southern Brazil. Fractionation of the ethanolic extract from Banara parviflora leaves afforded, a new compound identified as N 1,N 8-dibenzoylspermidinyl-N 4-acetamide, a spermidine alkaloid. The structure was elucidated by spectroscopic methods (IR, MS, 1H, 13C and 2-D NMR).

Lyroglossa e Pteroglossa(Orchidaceae: Spiranthinae) são registradas pela primeira vez para o Rio Grande do Sul. Descrições, ilustrações, notas taxonômicas e distribuição são apresentadas. Nós também fornecemos uma chave artificial para distinguir os gêneros do “Clado Stenorrhynchos” registrados até o momento para o Rio Grande do Sul.

Lyroglossa and Pteroglossa(Orchidaceae: Spiranthinae) are first recorded for Rio Grande do Sul. Descriptions, illustrations, taxonomic notes and distribution are presented. We also provide an artificial key to distinguish the genera of the “Stenorrhynchos Clade” reported so far in Rio Grande do Sul.

Um testemunho de sondagem coletado em Santo Antônio da Patrulha, Rio Grande do Sul, Brasil, foi submetido para análise polínica a fim de revelar a história vegetacional e mudanças paleoecológicas e paleoclimáticas. Um total de 98 táxons foi identificado a partir de 35 subamostras. Três datações radiocarbônicas foram obtidas ao longo de uma seção de 115 cm de profundidade, incluindo a idade basal de 4730 ± 50 anos AP. Diagramas polínicos e análises de agrupamentos foramrealizadas com base nas freqüências dos palinomorfos, demonstrando cinco fases distintas (SAP-I a SAP-V), as quais refletiram diferentes condições paleoecológicas. A predominância de plantas relacionadas à vegetação campestre na fase SAP-I sugere condições climáticas quentes e secas. Um gradual aumento nas condições de umidade foi observado principalmente no início da fase SAP-III, quando a vegetação conformou um mosaico de Campos e Floresta Atlântica. Além disso, a presença de certos táxons florestais (tipo- Acacia, Daphnopsis racemosa, tipo Erythrina e tipo Parapiptadenia rigida), a partir da fase SAP-IV, é interpretada como influência da Floresta Estacional Semidecidual na região de estudo. A partir da fase SAP-V (ca. 4000 anos AP) a vegetação tornou-se similar à moderna (atual Bioma da Floresta Atlântica), especialmente após 2000 anos AP (idade calibrada).

A sedimentar core collected at Santo Antônio da Patrulha, Rio Grande do Sul State, southmost Brazil, was submitted to pollen analysis to provide the vegetational history of this region, and the paleoecological and paleoclimatic changes. A total of 98 taxa of palynomorphs was identified from 35 subsamples. Three radiocarbonic datings were obtained along a section of 115 cm depth, including the basal age of 4730 ± 50 yr BP. Pollen diagrams and cluster analysis were performed based on palynomorphs frequencies, demonstrating five distinct phases (SAP-I to SAP-V), which reflected different paleoecological conditions. The predominance of plants associated with grasslands in the phase SAP-I suggests warm and dry climate conditions. A gradual increasing of humidity conditions was observed mainly from the beginning of the phase SAP-III, when the vegetation set a mosaic of grasslands and Atlantic rainforest. Furthermore, the presence of some forest taxa ( Acacia-type, Daphnopsis racemosa, Erythrina-type and Parapiptadenia rigida-type), from the phase SAP-IV, is interpreted as an influence of the seasonal semideciduous forest in the study region. From the phase SAP-V (ca. 4000 yrs BP), the vegetation became similar to the modern one (extant Atlantic rainforest Biome), especially after 2000 yrs BP (calibrated age).

Extratos clorofórmicos ou diclorometânicos de 357 espécies vegetais de ocorrência no sul do Brasil foram testados quanto à presença de peróxidos, através de cromatografia em camada delgada, utilizando o reagente de detecção de Huber & Fröhlke. Das espécies testadas, 71 (20%) apresentaram resultado positivo, a maioria delas (56%) pertencentes à família Asteraceae. A maioria das espécies testadas pertencia a Asteraceae, mas outras 55 famílias foram também testadas, abrangendo um total de 77 gêneros.

Chloroform or dichloromethane extracts of 357 southern Brazilian plant species were tested for the presence of peroxides by thinlayer chromatography, using the spray reagent from Huber & Fröhlke. From the species tested, 71 (20%) showed positive results and most of them (56%) are Asteraceae species. The species tested were mainly from Asteraceae, but 55 more families were screened, in a total of 77 genera surveyed.